Just some fun facts about S-scale.
Famous prototype photographer and author Jim Boyd
(source: Railroad Model Craftsman magazine, October 1978 issue, pg 76).
Well-known artistic modeler and author Malcolm Furlow
(source: Railroad Model Craftsman magazine, September 1978 issue, pg 102).
Silent-movie comedian and actor Buster Keaton
(source: Trains Magazine web site).
Well-known HO-scale model railroader and author Allen McClelland
(source: Railroad Model Craftsman magazine, March 1977, pg 44; shows layout photo).
Railroad Model Craftsman magazine contributor Mike Schafer
(source: Railroad Model Craftsman magazine, August 2015, pg 78).
World-famous HO-scale model railroader George Selios
(source: Mainline Modeler magazine, November 1995, pg 62).
Apparently the headquarters building of the Kansas City Southern Railway, located at 427 W 12th St, Kansas City, Missouri, has an S-scale display layout in their building.
The Kansas City Union Station, located at 30 Pershing Road, Kansas City, Missouri, has a multi-scale layout which features an S-scale section. There was some remodeling taking place in that area, and work continues on the hi-rail/scale S-scale layout. S-scale modeler Roy Inman volunteers there from time to time, so if you are in the area and want to help, contact the museum or Roy.
The Railroad Museum of South Florida is located at 7330 Gladiolus Drive, Fort Myers, FL 33908. It has a 9'x20' layout using MTH's track. See the May 2017 issue of the NASG's Dispatch to see some photos of the layout, its track plan, and some of the issues they had building the layout.
The Shelburne Museum in Vermont has an original American Flyer display layout set up on the second floor. The owner of the museum at the time was a friend of A.C. Gilbert, and built an A.F.-based layout in 1954 measuring 8'x20'. The layout changed hands several times over the years, but in 2004 it found its way back to the museum. The museum also has some prototype equipment on its property. A video of the current-day layout is available on YouTube. This one is older, but shows more close-up shots. The March 2019 issue of the NASG's Dispatch has an article about the museum's layout starting on page 15, with several additional photos and a track plan.
The Eli Whitney Museum and Workshop has an annual display of the A.C. Gilbert trains starting at Thanksgiving up through early January. Be sure to contact them before visiting, to see if they are still planning on having that exhibition this year. This is a video of a brief interview with its director.
The Lake Superior Railroad Museum in Duluth, Minnesota has Lee Jacques' S-scale layout preserved. The September 1980 issue of S Gaugian has an article about this layout, including photos. Lee died in 1969 and his wife donated the layout to the museum.
The National Toy Train Museum and Library in Strasburg, Pennsylvania has a two-level S-scale display layout. Follow the link to see an onboard video of each of the levels.
The Gadsden Pacific Toy Train Museum in Tucson, Arizona has a nice-sized American Flyer layout.
The Famous-Barr Christmas display layout maintained by the American Flyer S Gaugers of the St. Louis Area (AFSGSLA) club.
The Cincinnati Museum Center, in Cincinnati, Ohio Cincinnati in Motion display features an S-scale layout. In recent years it has been completely refreshed. You can see various videos of the previous and current layout online via: video #1, video #2, video #3, video #4.